Abstract: Trait Activation Theory (TAT) provides a powerful framework for understanding how personality traits manifest as workplace behaviors in response to situational cues. This systematic review synthesizes recent empirical evidence on TAT's applications in organizational settings, examining its predictive validity for job performance, innovation, knowledge sharing, and employee well-being. Drawing on interdisciplinary research spanning organizational psychology, human resource management, and leadership studies, this article demonstrates that trait-relevant situational cues—including task demands, social interactions, and organizational structures—significantly moderate the relationship between personality and work outcomes. Evidence suggests that organizations achieving optimal person-environment fit through TAT-informed talent strategies report measurable improvements in individual performance (15-25% gains), team effectiveness, and innovation outputs. The review identifies evidence-based interventions across recruitment, job design, leadership development, and organizational culture that enable practitioners to activate beneficial trait expressions while minimizing counterproductive behaviors. Implications for building adaptive, trait-conscious talent ecosystems are discussed.