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From seed to cDNA―lessons learned from plant RNA workflows

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Manage episode 508242388 series 3466726
Content provided by Thermo Fisher Scientific. All podcast content including episodes, graphics, and podcast descriptions are uploaded and provided directly by Thermo Fisher Scientific or their podcast platform partner. If you believe someone is using your copyrighted work without your permission, you can follow the process outlined here https://podcastplayer.com/legal.

In this Mol Bio Minutes episode, Dr. Will Barnes, a plant biologist turned senior sales training specialist at Thermo Fisher Scientific, reflects on his time at the bench and the molecular lessons learned along the way. Will dives deep into the difficulties of RNA work in plant systems—carbohydrate contamination, genomic DNA carryover, secondary structure—and how these hindered everything from cloning and expression analysis to sequencing and qPCR.

Through real-world anecdotes, he explains how high-quality reagents and systems helped him troubleshoot and resolve recurring issues, ultimately saving time and improving data reliability. He advocates for prioritizing upstream steps like RNA integrity and reverse transcription fidelity instead of chasing fixes at the end of the workflow.

Listeners will leave with a deeper appreciation for the critical role of sample prep in molecular biology workflows and why investing in better tools pays off in cleaner data, greater confidence, and fewer headaches. Will’s experience is a relatable reminder that smarter choices lead to better science.

Helpful resource links mentioned in this episode:

----------------------------------------------------------------------------------------------

Limited time promotional offer – Be one of the first 75 listeners to spend $500 and get 40% off all eligible reverse transcriptase, plastics, enzymes, and cloning reagents.

Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague. 

Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.

For Research Use Only. Not for use in diagnostic procedures.

  continue reading

40 episodes

Artwork
iconShare
 
Manage episode 508242388 series 3466726
Content provided by Thermo Fisher Scientific. All podcast content including episodes, graphics, and podcast descriptions are uploaded and provided directly by Thermo Fisher Scientific or their podcast platform partner. If you believe someone is using your copyrighted work without your permission, you can follow the process outlined here https://podcastplayer.com/legal.

In this Mol Bio Minutes episode, Dr. Will Barnes, a plant biologist turned senior sales training specialist at Thermo Fisher Scientific, reflects on his time at the bench and the molecular lessons learned along the way. Will dives deep into the difficulties of RNA work in plant systems—carbohydrate contamination, genomic DNA carryover, secondary structure—and how these hindered everything from cloning and expression analysis to sequencing and qPCR.

Through real-world anecdotes, he explains how high-quality reagents and systems helped him troubleshoot and resolve recurring issues, ultimately saving time and improving data reliability. He advocates for prioritizing upstream steps like RNA integrity and reverse transcription fidelity instead of chasing fixes at the end of the workflow.

Listeners will leave with a deeper appreciation for the critical role of sample prep in molecular biology workflows and why investing in better tools pays off in cleaner data, greater confidence, and fewer headaches. Will’s experience is a relatable reminder that smarter choices lead to better science.

Helpful resource links mentioned in this episode:

----------------------------------------------------------------------------------------------

Limited time promotional offer – Be one of the first 75 listeners to spend $500 and get 40% off all eligible reverse transcriptase, plastics, enzymes, and cloning reagents.

Subscribe to get future episodes as they drop and if you like what you’re hearing we hope you’ll share a review or recommend the series to a colleague. 

Visit the Invitrogen School of Molecular Biology to access helpful molecular biology resources and educational content, and please share this resource with anyone you know working in molecular biology.

For Research Use Only. Not for use in diagnostic procedures.

  continue reading

40 episodes

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