Pichia Pastoris: The Microbial Protein Expression Platform You’re Probably Underestimating Beyond Biotech

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Pichia pastoris: the microbial protein expression platform you’re probably underestimating

6d ago 36:02

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Today we’re diving into the world of expression systems with Iskandar Dib, of Validogen and we’ll explore the remarkable potential of Pichia pastoris, a methylotrophic yeast that’s revolutionizing pharmaceutical protein production and beyond.

From industrial enzymes to biomaterials, food, feed, and diagnostics, Pichia offers a reliable, scalable, and cost-efficient platform for recombinant protein expression.

Iskandar explains how biotechs can unlock Pichia’s full potential through Validogen’s sophisticated tools and strategies, fine-tuning protein expression with innovative genetic approaches and robust screening systems to deliver unmatched yields and quality.

01:06 Why Pichia is often overlooked or underestimated

03:29 The basics of Pichia pastoris

05:25 How Pichia compares to microbial hosts like E.coli

08:14 Key advantages over mammalian cell systems

10:21 Pichia as a system for secreted production

11:30 Pichia and post-translational modifications

14:30 The challenges of working with Pichia

16:25 Strain optimization and genetic engineering

17:29 Meet Iskandar Dib and Validogen

19:03 The UNLOCK PICHIA platform

24:00 Real-world applications of Pichia pastoris in biopharma

27:19 When Pichia outperforms expectations

28:25 Adapting Pichia for emerging fields

30:23 Advice for those considering Pichia pastoris for protein expression

33:23 Learn more about Validogen

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To dive deeper into the topic:

Chapters

1. Pichia pastoris: the microbial protein expression platform you’re probably underestimating (00:00:00)

2. Why pichia is often overlooked or underestimated (00:01:06)

3. The basics of pichia pastoris (00:03:29)

4. How pichia compares to microbial hosts like E.coli (00:05:25)

5. Key advantages over mammalian cell systems (00:08:14)

6. Pichia as a system for secreted production (00:10:21)

7. Pichia and post-translational modifications (00:11:30)

8. The challenges of working with pichia (00:14:30)

9. Strain optimization and genetic engineering (00:16:25)

10. Meet Iskander Dib and Validogen (00:17:29)

11. The UNLOCK PICHIA platform (00:19:03)

12. Real-world applications of pichia pastoris in biopharma (00:24:00)

13. When pichia outperforms expectations (00:27:19)

14. Adapting pichia for emerging fields (00:28:25)

15. Advice for those considering pichia pastoris for protein expression (00:30:23)

16. Learn more about Validogen (00:33:23)

170 episodes

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