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Imaging GPCRs in Action: Chemical Probes for Next-Level Discovery | Dr. Johannes Broichhagen #178

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Manage episode 522378734 series 2685422
Content provided by Dr. Yamina Berchiche. All podcast content including episodes, graphics, and podcast descriptions are uploaded and provided directly by Dr. Yamina Berchiche or their podcast platform partner. If you believe someone is using your copyrighted work without your permission, you can follow the process outlined here https://podcastplayer.com/legal.

Chemical probes are reshaping how we map GLP-1R in real time — revealing receptor pools antibodies can’t reliably capture.

This is Episode 2 of a 3-part GPCR tool-development series created in partnership with Celtarys Research.

Summary:

Dr. Johannes Broichhagen aka JB breaks down the design logic behind fluorophore-linked peptides, assay trade-offs, and what true receptor internalization looks like in live tissue. A concise masterclass in assay development and GPCR drug discovery.

What you’ll learn:

• Why antibody variability pushed JB toward chemical probe engineering

• The design logic behind Luxendin-based fluorescent tools — and how structure guides function

• What “good assay development” looks like when cells, tissue, and probe behavior collide

• Behind-the-scenes stories from the collaboration with David Hodson

• Why parallelized experiments matter for reproducibility and signal quality

• How small-molecule probes outperform antibodies in live-cell and tissue imaging

• The surprising breakthroughs that shifted JB’s entire research trajectory

• Future directions: multi-color GPCR mapping, AI-guided ligand design, and in vivo chemical biology

Dr GPCR Links & Resources:

• Dr. GPCR Ecosystem: https://www.ecosystem.drgpcr.com/

• Membership & Pricing: https://www.ecosystem.drgpcr.com/university-pricing• Weekly News: https://www.ecosystem.drgpcr.com/gpcr-weekly-news

  continue reading

216 episodes

Artwork
iconShare
 
Manage episode 522378734 series 2685422
Content provided by Dr. Yamina Berchiche. All podcast content including episodes, graphics, and podcast descriptions are uploaded and provided directly by Dr. Yamina Berchiche or their podcast platform partner. If you believe someone is using your copyrighted work without your permission, you can follow the process outlined here https://podcastplayer.com/legal.

Chemical probes are reshaping how we map GLP-1R in real time — revealing receptor pools antibodies can’t reliably capture.

This is Episode 2 of a 3-part GPCR tool-development series created in partnership with Celtarys Research.

Summary:

Dr. Johannes Broichhagen aka JB breaks down the design logic behind fluorophore-linked peptides, assay trade-offs, and what true receptor internalization looks like in live tissue. A concise masterclass in assay development and GPCR drug discovery.

What you’ll learn:

• Why antibody variability pushed JB toward chemical probe engineering

• The design logic behind Luxendin-based fluorescent tools — and how structure guides function

• What “good assay development” looks like when cells, tissue, and probe behavior collide

• Behind-the-scenes stories from the collaboration with David Hodson

• Why parallelized experiments matter for reproducibility and signal quality

• How small-molecule probes outperform antibodies in live-cell and tissue imaging

• The surprising breakthroughs that shifted JB’s entire research trajectory

• Future directions: multi-color GPCR mapping, AI-guided ligand design, and in vivo chemical biology

Dr GPCR Links & Resources:

• Dr. GPCR Ecosystem: https://www.ecosystem.drgpcr.com/

• Membership & Pricing: https://www.ecosystem.drgpcr.com/university-pricing• Weekly News: https://www.ecosystem.drgpcr.com/gpcr-weekly-news

  continue reading

216 episodes

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