Best Cytometry Podcasts (2025)

1
Anis Larbi (Beckman Coulter Life Sciences) 1:01:39

5d ago1:01:39

1:01:39

In this episode of Flow Stars, we're joined by Anis Larbi, Medical & Scientific Affairs, Beckman Coulter Life Sciences. Anis chats about his experience working at the prestigious A*STAR in Singapore, where he led a dual career as both a principal investigator and the head of a core flow cytometry facility. He also shares his advocacy of networking,…

1
Sofie Van Gassen (VIB-UGent) 53:44

19d ago53:44

53:44

In this episode, Peter O'Toole welcomes Sofie Van Gassen, a Postdoctoral Researcher from VIB-UGent. Sofie explains how a computer scientist can end up working in flow cytometry and sets out her vision for the future of data science in flow. She also sets out her twin passions for developing algorithms and pipelines for data analysis—and gardening. …

1
David Novo-Lake (De Novo Research) 1:06:17

1M ago1:06:17

1:06:17

Today, Peter O'Toole chats with Dave Novo-Lake, founder of De Novo Research, a pioneering figure in flow cytometry data analysis, and the creator of FCS Express. Dave shares his journey from developing groundbreaking software in his spare time at a Boston lab to establishing a successful business that transformed clinical flow cytometry. He also ta…

1
Building on Backbone Panels: Standardization, Speed, and Integration 1:03:40

1M ago1:03:40

1:03:40

In the 19th episode of “The Conversational Flow”, our hosts, Brian and Adam, dive into the utility and innovation behind backbone panels in spectral flow cytometry, spotlighting a recent paper from BMS that presents a robust, flexible assay designed for immune monitoring using the Aurora platform. They discuss how backbone panels streamline assay d…

1
Mario Roederer (NIH) 54:06

2M ago54:06

54:06

Today, our host Peter O'Toole sits down with the distinguished Mario Roederer, ImmunoTechnology Section Chief and Acting Head of the Translational Research Program at the National Institutes of Health. Mario shares some fascinating insights from his illustrious career, from demonstrating flow cytometry to Presidents Barack Obama and George Bush to …

1
Hepatocyte Counting Methods: From Manual Counts to Fast, Accurate Automation 17:29

2M ago17:29

17:29

#115 — Hepatocytes are liver cells that are essential for its function. They are also essential for certain branches of drug testing and diagnostics. However, they have irregular shapes, multiple nuclei, and can clump, which makes them difficult to process and count accurately. In this episode, we explain how to prepare the best hepatocyte samples …

1
Spectral Flow Cytometry Special 1:06:39

2M ago1:06:39

1:06:39

Let's shift our focus from just the people behind the science to the technologies they're using. In this special episode, we dive deep into spectral flow cytometry with a panel of leading experts, featuring: • Tamar Tak, Coordinator Flow Cytometry Facility, Leiden University Medical Center • Domenico Lo Tartaro, University of Modena and Reggio Emil…

1
Single Cell Sequencing: Tips to Optimize Nuclei Extraction and Counting 16:58

2M ago16:58

16:58

#114 — High-quality nuclei extraction is pivotal for many genetic and epigenetic studies, including single cell RNA-seq to describe transcriptomic profiles and gene expression dynamics and ATAC-seq to determine chromatin accessibility. But nuclei samples are extremely delicate and can be challenging to prepare and count. In this episode, we provide…

1
Flow Cytometry & Cell Therapy: One Cell Can Make a World of Difference 1:08:51

2M ago1:08:51

1:08:51

In this engaging episode of the podcast, hosts Brian Wile and Adam Cotty welcome special guests Michael Naso, Hillary Quinn, and Barry Morse from The Therapeutic Innovations Group—a powerhouse team of scientists and biotech veterans with decades of experience across biologics, small molecules, and cell therapy. The conversation dives deep into the …

1
Flow Validations and Guidance Document Changes in 2024-2025 45:48

3M ago45:48

45:48

Episode 17 of The Conversational Flow podcast, hosted by Brian Wile and Adam Cotty, dives into the evolving landscape of flow cytometry networking and regulatory guidance, where they analyze recent FDA biomarker validation guidance, comparing it to existing industry standards like CLSI H62. Our hosts explore the challenges of harmonizing flow cytom…

1
The Role of Flow in Drug Autoimmune Indication Research 1:00:41

4M ago1:00:41

1:00:41

In episode 16 of The Conversational Flow podcast, hosts Brian Wile and Adam Cotty are joined by Allie Greenplate and Ayman Rezk to explore “The Role of Flow in Drug Autoimmune Indication Research.” The discussion delves into the surge in pharmaceutical R&D for immune and autoimmune indications, highlighting the influence of oncology treatments in s…

1
Fantastic Fluorophores and Where to Find Them (w/ Kelly Lundsten) 1:05:35

5M ago1:05:35

1:05:35

In this exciting 15th episode of The Conversational Flow podcast, titled "Fantastic Fluorophores and Where to Find Them," hosts Brian Wile and Adam Cotty from KCAS Bio are joined by special guest Kelly Lundsten from FluoroFinder. Together, they dive into the fascinating world of spectral flow cytometry, exploring key questions such as whether spect…

1
Modifying Flow Cytometry Panels: the “Backbone” of Flow Cytometry 50:27

6M ago50:27

50:27

A flow cytometry backbone panel is a set of antibodies that can be used as a starting point for identifying cell types, and then supplemented with additional markers. On the most recent episode of “The Conversational Flow”, our hosts, Brian and Adam, spend some time together as they answer the questions of “What is a backbone panel?”, “What type of…

1
Personal Advice on How to Respond to Criticism of Your Grant Proposal Reviews 13:15

7M ago13:15

13:15

#113 — Learning how to respond to criticism is an unavoidable aspect of grant applications. Follow all instructions from funding agencies to avoid immediate rejection. This episode of Mentors At Your Benchside gives you some simple strategies to ensure you respond to criticism in a constructive and proportionate way. For example, identifying review…

1
Low Content Analysis: When a smaller panel is better than a larger one 1:04:14

7M ago1:04:14

1:04:14

During the most recent episode of “The Conversational Flow” podcast, our hosts Brian and Adam talk about those times when a smaller panel might be desirable over a larger panel. To dive deep into this discussion and really analyze all the angles of these questions, the guys have invited a special guest to join them – Amrutesh Puranik, PhD. Amrutesh…

1
High Content Analysis: Finding Needles in a Growing Haystack 1:03:05

8M ago1:03:05

1:03:05

In the 12th episode of “The Conversational Flow” podcast, Brian and Adam welcome two special guests – Sean Burke of Denovo Software and Ryan Brinkman of Dotmatics – who join them to discuss the answers to a number of specific questions about High Content Analysis: How can we manage and derive insight from higher complexity panels? How can this be s…

1
Isoelectric Focusing: A Simple Way to Enhance Your Protein Separation 9:20

9M ago9:20

9:20

#112 — Isoelectric focusing (IEF) is a powerful technique distinct from the more familiar SDS-PAGE, [1,2] tailored for separating proteins or peptides based on their isoelectric points (pI). [3] This method capitalizes on the migration of charged molecules through a stable pH gradient until they reach a zone where their net charge is zero, halting …

1
Going Global with Spectral Flow Cytometry - US, EU and Now Australia 1:06:10

9M ago1:06:10

1:06:10

During this special episode of "The Conversational Flow" our hosts, Brian and Adam, welcome two guests: John Bucksath, CEO of KCAS Bio, and Stefan Cross, CEO of Crux Laboratories. These two guests discuss the elements and advantages of their organizations' new partnership between KCAS' US and European locations and Crux's location in Melbourne, Aus…

1
Top 10 Uses of Microbes in Biotechnology 9:09

10M ago9:09

9:09

#111 — In this episode, we dive deep into the fascinating world of microbes and their revolutionary applications in biotechnology. From environmental solutions to breakthroughs in health and medicine, microbes hold the key to some of the most advanced scientific developments. Discover how these microscopic organisms are transforming industries and …

1
Let’s Talk About Stats: Methods for Comparing Two Sets of Data 4:32

10M ago4:32

4:32

#110 — Comparing two sets of data is a fundamental process in statistical analysis, crucial for drawing meaningful conclusions across various fields. Whether it's for determining the success of an intervention, understanding market trends, or validating scientific research, the need for comparison arises. This episode delves into the essence of dat…

1
Instrument Comparison: Some Instruments are More Equal than Others 55:01

10M ago55:01

55:01

When it comes to flow cytometry instrumentation, there are a number of things that can be different. You may have different types of lasers, you might have a different path for the laser to follow to get to the detector, you can have different actual detectors, different filters in front of those detectors, not to mention the varying sensitivities …

1
Flow Stars Recorded Live at CYTO 2024 34:59

10M ago34:59

34:59

In this special episode of Flow Stars recorded live at CYTO 2024, our panel of esteemed guests, representing a cross-section of cytometry users, discuss the biggest matters in flow cytometry. They explore the capabilities of current cytometers and what future developments should focus on. They also consider the importance of making cytometry equipm…

1
Personal Advice on Building Your Professional Network. It Takes a Village 10:45

11M ago10:45

10:45

#109 — How do you build a scientific network that gives you the best chance of getting your research funded? How can you identify who to include in your network, and how should you contact them? This episode explains how to build a scientific network that works for you. We discuss the answers to these questions and provide some examples of collabor…

1
4 Fixatives for Histology and Cytometry: Perfect Your Preservation 6:55

11M ago6:55

6:55

#108 — What should you use to fix your cells? Alcohols or aldehydes? Gluteraldehyde or formaldehyde? And how long will your cells stay fixed? This episode explains the four main fixatives for histology and cytometry and when to use them. It also provides some practical tips to ensure your fixation works and explains the benefits of combining fixati…

1
Batch Effects, Controls, and Considerations for Longitudinal Studies 1:04:56

11M ago1:04:56

1:04:56

Let’s face it - nobody likes batch effects (unless it has to do with cookies, but that’s for another podcast). In episode #9 of “The Conversational Flow”, Brian and Adam talk about Batch Effects which is when you're having changes in the study that are not necessarily related to the scientific variables of the study – and what that might mean you w…

1
Microscope Disinfection: A Quick Guide 6:30

11M ago6:30

6:30

#107 — Need to give your microscope a quick clean to get rid of some grime but unsure what cleaning agent to use? Have you had a nasty sample on there recently and need to disinfect it for the next user? This episode gives you a quick guide to disinfecting your microscopes, including what solvents are safe to use and the parts you should tackle fir…

1
Tissue Processing For Histology: What Exactly Happens? 6:11

12M ago6:11

6:11

#106 — Transforming a tissue sample into a slide ready for microscopic exploration involves a series of critical steps. Among these, tissue processing is a fundamental phase bridging tissue fixation and the embedding/sectioning of paraffin blocks. In this episode, discover what exactly happens in this vital in-between stage, and learn about the six…

1
Multiple Fragment Ligation: The Why and How 7:24

1y ago7:24

7:24

#105 — You may be familiar with standard single fragment ligations: insert, vector, ligase—done! But what if you have a complex cloning project with a massive region of DNA to clone? You can’t PCR the whole thing, and you can’t cut the entire thing out from somewhere else. What do you do? In this episode, we explain the answer: multiple fragment li…

1
Receptor Occupancy Testing in Clinical Trials 45:36

1y ago45:36

45:36

For episode #8 of “The Conversational Flow” podcast, Brian and Adam spend the time discussing Receptor Occupancy Testing in Clinical Trials. Receptor occupancy (RO) assays are important in clinical trials because they provide valuable information about a drug’s binding characteristics and mechanism of action. Our hosts begin by outlining the genera…

1
Funding Opportunities and the Flow of Money in Science 9:47

1y ago9:47

9:47

#104 — What funding stream is right for you? Industry or government? Non-profits or crowdfunding? It depends on what you're researching, but also where you want to take your career. In this episode, Joel Berry, Founder, and Chief Scientist at Astound Research, breaks down the different funding streams and flow of money in bioscience research. Disco…

1
Maxam–Gilbert Sequencing: What It Is and 3 Modern Applications 8:38

1y ago8:38

8:38

#103 — DNA sequencing is a fundamental technique in modern molecular biology that has revolutionized the study of genes. In the old days, Maxam–Gilbert sequencing was the method of choice, but it has mostly been replaced by Sanger sequencing and Next-Generation methods. Yet, it still has some niche uses, and in the historical context of DNA sequenc…

1
The Current State of Receptor Occupancy Validation 1:00:34

1y ago1:00:34

1:00:34

For episode #7 of “The Conversational Flow”, Brian and Adam invite David Ambrose to join them as they continue their conversation about Receptor Occupancy assays. There are so many unique considerations around an RO assay and with any flow cytometry assay, the precision of that assay is important. Our hosts discuss the biggest differentiators when …

1
Confocal Laser Scanning Microscopy Explained In 3 Easy Steps 8:07

1y ago8:07

8:07

#102 — Fluorescence microscopy images not only look great but also allow us to get a better understanding of cells, structures, and tissues. And confocal laser scanning microscopy lets us construct 3D images from 2D micrographs. In this episode, learn the basic principles of confocal laser scanning microscopy, how the microscopes work, and some of …

1
How to Become an Expert at Getting Funded 10:05

1y ago10:05

10:05

#101 — Discover what it takes to become an expert at getting funded, from simple habits such as summarizing what you read in the literature, to big steps such as organizing your very own conference to establish your name in your field. With over 30 years of experience as a biomedical engineering researcher seeking grants, Joel Berry, Founder, and C…

1
Receptor Occupancy: Deceptively Simple Questions 38:05

1y ago38:05

38:05

The newest episode of “The Conversational Flow” has Brian and Adam talking about the concepts around Receptor Occupancy and all the deceptively simple questions people often have about them, as well as the important but sometimes difficult answers to those questions. They talk about the biology of receptors and the few different "classes" associate…

1
5 Types of Difficult Lab Supervisor and How to Handle Them 8:28

1y ago8:28

8:28

#100 — Science attracts so many different and quirky personalities that you are bound to have some people you just don’t get along with. Conflicts happen, and there are many strategies you can take to deal with conflict in the lab. But when your lab supervisor is the problem, it can be a big issue for you. In this episode, delve into the challenges…

1
7 Top Tips to Make the Most of Your Flow Cytometry Training 12:35

1y ago12:35

12:35

#99 — So you’ve got your flow cytometry training booked and are one step closer to that precious data. But if you want to hit the ground running and get some useful data from your samples, there are some little things you'll need to do. These include reading up on a bit of background theory, understanding the capabilities of different types of cyto…

1
What Reagents Can You Use Past Their Chemical Expiry Date? 10:04

1y ago10:04

10:04

#98 — Our labs can contain thousands of chemicals, many of which will be past their given expiry date and many of which are expensive to buy and replace. Replacing them when you don't need to can be a waste of time and grant money. On the other hand, using expired chemicals can lead to failed experiments and confusing results. In this episode of Me…

1
What Is Cell Sorting and What Role Does It Play in Bioanalysis? 44:13

1+ y ago44:13

44:13

During this newest episode of “The Conversational Flow”, Brian and Adam discuss cell sorting and the pivotal role it plays in molecular biology applications – enabling researchers to isolate specific cell populations from complex mixtures, as well as a number of other benefits. Our hosts dive into the importance of cell sorting, the evolution of th…

1
How to Write an Effective Research Interest Statement 7:21

1+ y ago7:21

7:21

#97 — A research interest statement is essential to successfully apply for an academic job. In this episode, we delve into how to craft an outstanding one. [1] We cover strategies to outline your past, current, and future research in a concise format. We also explain other key elements such as, creating a compelling introduction, detailing research…

1
How to Preserve Microorganisms: Store Your Cells Better 7:33

1+ y ago7:33

7:33

#96 — An appropriate microorganism preservation method can make all the difference in maintaining the viability of your microbial strains because it plays a crucial role in ensuring reproducible results and continuity in research. In this episode, learn the preservation methods for short- and long-term microbe storage, their pros and cons, and the …

1
Overhang PCR: Add Missing DNA Sequences Using Primers 5:42

1+ y ago5:42

5:42

#95 — Have you ever accidentally forgotten to add the Kozak consensus sequence to the start of a coding gene? Or forgotten to include the stop codon? Did you clone something, then realize you wanted to tag it with something? Or do you want to add restriction enzymes to your PCR product to make it easier to clone into a plasmid? Overhang PCR may be …

1
Practical Applications and Considerations of Phenol-Chloroform Extraction 12:06

1+ y ago12:06

12:06

#94 — While there are lots of methods to choose from for cleaning up your RNA or DNA samples, for many researchers, phenol-chloroform is the go-to technique. In this episode, go beyond the basics of how the method works and get expert practical guidance on performing and optimizing it. Plus, learn the differences between the common solvents, how to…

1
How to Become a Bioinformatician 5:14

1+ y ago5:14

5:14

#93 — Bioinformatics is an interdisciplinary field that combines mathematics, computer science, physics, and biology to help answer key questions in modern biological sciences research. In this episode, we’ve got the lowdown on the training you’ll need to pursue this career path, and a handy list of resources to get you started on your learning. [1…

1
What Makes Spectral Flow so Significant? 46:15

1+ y ago46:15

46:15

For the 4th episode of “The Conversational Flow” podcast, Brian and Adam discuss spectral flow; something really near and dear to their hearts. This is a topic both of our hosts have been genuinely interested in since they really became aware of it, at which time they immediately began pushing to bring the instrumentation to KCAS. While they have h…

1
8 Cell Lysis Methods to Break Cell Walls 7:48

1+ y ago7:48

7:48

#92 — We all need to lyse cells to extract the goodness—our samples—from them. However, there are many cell lysis methods. Some are harsh, while some are gentle. Some are laborious, while some are easy. Some require dedicated equipment, while some do not. So which one do you choose? In this episode, we cover eight cell lysis methods for your experi…

1
Genetic Variants Explained 11:50

1+ y ago11:50

11:50

#91 — Genomes are complex and encode a vast quantity of information. One of their key features is genetic variants—aberrations in the genetic sequence, usually in the form of insertions, deletions, repeats, and translocations of genetic material. This episode explains the different types of genetic variants, introduces their key features, and gives…

1
How to Identify Supercoils, Nicks and Circles in DNA Plasmid Preps 4:28

1+ y ago4:28

4:28

#90 — Are you confused about the banding pattern of DNA on agarose gels? DNA can take many structural forms depending on its source and how you have isolated and purified it. And those forms, including linear, nicked, closed circled, and supercoiled, all migrate at different rates on agarose gels. But how do you identify which band corresponds to w…

1
How do You Validate a Flow Cytometry Assay? 43:47

1+ y ago43:47

43:47

There are a number of different guidances for Flow Cytometric Assay Validation, so for episode #3 of “The Conversational Flow” podcast, Brian and Adam go into great detail on how we perform validations at KCAS, and under what context we do so – and why. In this episode, the guys break down the different types of validation and the various levels of…

1
Simplicity in Science: How to Increase your Research Effectiveness by Doing Less 6:33

1+ y ago6:33

6:33

#89 — "Achieve more by doing less" sounds like a piece of cheap advice, but there is a lot of wisdom in it. Research is complicated. You must choose the best questions to ask, techniques, controls, organisms, and equipment, to name just a few things that make up good experiments. With so much to focus on, it becomes harder to do each of these thing…

Podcasts Worth a Listen

Cytometry Podcasts

Podcasts Worth a Listen